ABSTRACT
Objective:To establish a characteristic spectrum to reflect the efficacy of Houpo Qiwutang. Method:Based on the correlation between the efficacy and the pharmacological action of each herb in prescription，the target substances of characteristic map were screened. The extraction solvent，detection wavelength and gradient of the active ingredients were optimized. Peak assignment was made by comparing individual drugs. Q-TOF was used to infer the molecular formula of each peak in the characteristic atlas，and the reference substance was identified by the reference substance. The reference substance was screened out according to the correlation of main efficacy and medicine. Result:The characteristic spectrum of material standard of Houpu Qiwutang was established. Five of the seven herbal medicines were attributed. Nine characteristic peaks were selected and identified by Q-TOF as glycyrrhizin，including naringin，neohesperidin，ammonium glycyrrhizinate，rhein，honokiol，magnolol. According to the main efficacy of Houpo Qiwutang，neohesperidin was selected as reference substances. According to the separation of characteristic peaks and the retention time，the mark peak of the characteristic spectrum was determined. Conclusion:The characteristic spectrum of the material basis of Houpo Qiwutang was established by selecting the characteristic peaks and controlling the key components. This method not only reflects the situation of all the effective chemical components，but also focuses on the control of the key efficacy，so as to provide a theoretical basis for the subsequent development and quality control of Houpu Qiwu Tang.